Human Karyotyping Laboratory

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Male koryotype

Below is an example of human karyotyping.( Note: before you perform this activity you must read first the background.)

Background:

    Sometimes chromosomal is lost or rearranged during the formation of gametes or during cell division of the early embryo. Such changes, primarily the result of non dis junction or 

trans location, these are so severe that the pregnancy ends in miscarriage.

       Karyotype is the chromosomal characteristic of cell. It can show prospective whether they have certain abnormalities that could be passed on to their offspring ,or it might be used to lean caused child disability. Karyotype can also reveal certain defects through examination of cells from uterine fluid. 

   To create a karyotype, chromosomes from a cell are stained and photograph.This photograph will be enlarged and cut up into individual chromosomes.(Cornet's Living Environment Laboratory)

 Aim:

The aim of this laboratory experience is understand what is karyotype and how it is perform.

 Materials:
    Scissor
    Ruler
    Glue or paste
    Small envelope

 Procedure:

       1. Using the attached sheets, complete two different type of      karyotypes: normal female and male. (work slowly and carefully).

   

      2. Using scissors cut out the chromosome one by one and find it's exact match elsewhere on the page and paste both chromosomes side by side on " data page " that has heading filled out. Continue this procedure until you have matched all the chromosomes and paste each of them in the corresponding place on the data page place.( Note: Do not cut all chromosomes and them attempt to match them; cut only one at a time  you will lose it.)Use an envelope to store any chromosomes, so that it will not lose.

 Chromosomes Picture #1

 Chromosomes Picture #2